Medical Express

ISSN (print): 2318-8111

ISSN (online): 2358-0429

Author's Articles

4 result(s) for: Eliete Bouskela

Protective microcirculatory and anti-inflammatory effects of heparin on endotoxemic hamsters

Marcos L. Miranda; Luiz Felipe M. Prota; Maria Júlia B. Silva; Fernando L. Sicuro; Eliane S. Furtado; Ana Olimpia M. T. Santos; Eliete Bouskela


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OBJECTIVE: Apart from its anticoagulant properties, heparin has vasodilator and anti-inflammatory effects that could assist in the reversal of septic microcirculatory changes. This paper investigates the effects of heparin on endotoxemia-related microcirculatory changes and compares them to those observed with the use of recombinant human activated protein C.
METHODS: After skinfold chamber implantation procedures and endotoxemia induction by intravenous Escherichia coli lipopolysaccharide administration (2, male golden Syrian hamsters were treated with intravenous unfractionated heparin (0.2 Intravital microscopy of skinfold chamber preparations allowed quantitative analysis of microvascular variables and venular leukocyte rolling and adhesion. Macrohemodynamic parameters were also analyzed. Endotoxemic hamsters treated with recombinant human activated protein C and non-treated animals served as controls.
RESULTS: Heparin decreased lipopolysaccharide-induced leukocyte rolling and arteriolar vasoconstriction; it also increased survival when compared with non-treated animals, while recombinant human activated protein C decreased leukocyte adhesion. Administration of heparin plus recombinant human activated protein C was associated with a significant attenuation of lipopolysaccharide-induced capillary perfusion deficits.
CONCLUSIONS: Heparin yields protective effects on endotoxemic animals' microcirculation. Those benefits were potentiated when heparin was administered in conjunction with recombinant human activated protein C.

Keywords: sepsis; endotoxemia; microcirculation; heparin; recombinant human activated protein C.

Low dose of green tea catechins improves endothelial function and vascular smooth muscle cell reactivity in obese women

Daniel Alexandre Bottino; Débora Cherfan Goulart Nogueira; Ana Cláudia Lourenço; Vanessa Silveira Fortes; Andresa A. Berretta; Eliete Bouskela


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BACKGROUND: The high prevalence of obesity in the world is associated with several health problems, with endothelial dysfunction figuring as a frequent feature. We investigated whether low dose consumption of green tea extract (catechins < 200 mg/day) could modify endothelial function, lipid profile, fasting glucose and insulin, post load plasma glucose, inflammatory/oxidative stress biomarkers and blood pressure in obese women.
METHODS: Sixteen obese women with body mass index (BMI) between 30 and 40 Kg/m2, mean age 38 [33-40] years, consumed 600 ml green tea (3 × 200 ml) per day, containing 153.3 mg of catechins and 72.5 mg of caffeine, during three months. Endothelial function was evaluated through venous occlusion plethysmography by increment of peak forearm blood flow (FBF), after 5 min ischemia, during the reactive hyperemia response/baseline FBF. Endothelium-independent vasodilation was analyzed through peak FBF after 0.4 mg sublingual nitroglycerin/baseline FBF.
RESULTS: After 3 months, this consumption of green tea reduced BMI from 34.02 to 33.13, and diastolic blood pressure by 4 mmHg. The reactive hyperemia response/baseline FBF improved by 27%, and the endothelium-independent vasodilation by 12%. The blood biochemical profile, where all parameters were within the normal range, remained unaltered.
CONCLUSIONS: A low dose of green tea ameliorated the endothelial dysfunction present in obesity, indicating that its consumption should be encouraged in these patients, because endothelial dysfunction is an early marker of atherosclerosis.

Keywords: Obesity; green tea; microcirculation; venous occlusion plethysmography; endothelial function.

Vascular and Inflammatory Acute Responses after a Resistance Exercise Session in Young Women with Excessive Adiposity

Luiz Guilherme Kraemer-Aguiar; Belmiro Freitas de Salles; Ingrid Dias; Silvio Rodrigues Marques-Neto; Artur E. C. Guimarães; Maria G. C. de Souza; Braulio Santos; Eliete Bouskela


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BACKGROUND: Endothelial dysfunction and low-grade inflammation are both positively associated to states of excessive adiposity but reports on the acute effects of resistance exercise on these variables are still lacking. We evaluated these acute effects of resistance exercise on vascular reactivity and on the inflammatory profile in young women.
METHODS: Participants were divided into two groups: lean Controls (n=16) and Overweight (n=16). The resistance exercise session consisted of unilateral elbow flexions for five sets of 10 repetitions at 70% of one repetition maximum. Blood pressure, heart rate, forearm blood flow, vascular conductance, cytokines, adipopeptides and endothelin-1 were evaluated at rest and during the acute post-exercise period.
RESULTS: The overweight group had higher forearm blood flow at rest (p=0.03) and during post-exercise (p<0.001) while forearm vascular conductance was higher only during post-exercise, at 20 (p=0.02) and 40 min (p<0.001). Endothelial-dependent vasodilation was higher during the post-exercise period in the Overweight group compared to controls (p=0.01). In the Overweight group, the resistance exercise session reduced interleukin-6 (p=0.02) and leptin (p<0.001) but increased endothelin-1 levels (p=0.02).
CONCLUSIONS: We conclude that the single resistance exercise session elicited an acute increment of baseline vascular reactivity and an increased endothelial-dependent vasodilation with concomitant changes in inflammatory profile and endothelin-1 in our tested women with excessive adiposity.

Keywords: metabolic syndrome, exercise , inflammation.

Obesity and gut microbiota - what do we know so far?

Vicente Lopes da Silva-Junior; Fernanda de Azevedo Marques Lopes; Rodolpho Mattos Albano; Maria das Graças Coelho de Souza; Carolina Monteiro de Lemos Barbosa; Priscila Alves Maranhão; Eliete Bouskela; Luiz Guilherme Kraemer-Aguiar


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In the history of medicine, only recently has obesity been recognized as a disease. We know now that it is a pandemic condition, partly explained by the so-called Western lifestyle and related to multiple other comorbidities in various systems. This lyfestyle includes eating large portions, rich in saturated fats and refined sugar, all coupled with sedentary habits. In recent years, the gut microbiota has been indited as a new culprit in pathophysiological aspects involved in obesity. From studies with animals free of bacteria in the digestive tract, known as "germ-free animals"; the relevance of intestinal microbiota in the regulation of body fat became evident and its importance has also been extended to the pathophysiology of diseases such as diabetes mellitus and coronary heart disease. Characterization of Toll-like receptors led to the discovery of mechanisms that link the immune system with some metabolic pathways and opened new avenues of a previously unknown world to biological sciences. Increased knowledge about interactions between gut microbiota and the host can certainly reveal, in a not too distant future, new therapeutic perspectives for obesity and its related diseases.

Keywords: obesity; gut; microbiota